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KMID : 0616120170480010018
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Korean Journal of Pharmacognosy
2017 Volume.48 No. 1 p.18 ~ p.24
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Anti-inflammatory Activity of Essential Oil Extracted from Chamaecyparis obtusa (Sieb. et Zucc.) Wood
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Yang Ji-Yoon
Ahn Chang-Hwan
Choi Won-Sil
Kim Jae-Woo
Park Mi-Jin
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Abstract
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This study was conducted to determine the anti-inflammatory effect of essential oil extracted from the wood of Chamaecyparis obtusa Sieb. et Zucc. Endl. (Cupressaceae). The essential oil was extracted from the wood of C. obtusa by hydrodistillation method, and conducted the analysis on the chemical composition of the extracted C. obtusa wood oil through GCMS. The major constituents of the oil were found to be: α-pinene (11.4%), cadinene (5.4%), δ-cadiene (9.0%), τ-muurolol (22.2%), α-cadinol (20.8%) etc. We attempted to identify the anti-inflammatory activities of the oil when it is injected in the lipopolysaccharide (LPS)-stimulated RBL-2H3 cells, along with its effects on the secretion of interleukin-4 (IL-4), interleukin- 13 (IL-13), β-hexosaminidase. According to the cell viability analysis conducted by MTT assay, the oil in 10 -7 ~10 -5 % concentration showed no effect on the cell viability. After RBL-2H3 cells treated by LPS stimulation were exposed to 10 -7 % concentration of C. obtusa wood oil, the expression levels of IL-4, IL-13 within the cell were observed to remarkably decrease. Also, it was attenuated the release of β-hexosaminidase from mast cells to a significantly meaningful level. These results suggest that C. obtusa wood oil exerts the anti-inflammatory effect, by regulating the expression of inflammatory cytokines, which is a valuable feature to be highly utilized as the functional materials in the future.
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KEYWORD
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Chamaecyparis obtusa, Essential oil, Anti-inflammatory, Interleukin, β-Hexosaminidase
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